PrP genotypes and experimental scrapie in orally inoculated Suffolk sheep in the United States.
نویسندگان
چکیده
One-hundred and three Unites States Suffolk sheep were inoculated orally with a scrapie agent preparation and monitored for clinical disease and histopathological lesions characteristic of scrapie. A retrospective study of the polymorphisms at codon 171 of the prion protein (PrP) gene was performed on these sheep. All 63 sheep that developed scrapie during the observation period were homozygous for the glutamine 171 (171-QQ) PrP allele. Twelve 171-QQ sheep failed to develop disease. All 5 sheep homozygous for arginine (171-RR) and all 23 heterozygous (171-QR) sheep remained free of scrapie.
منابع مشابه
Experimental oral transmission of United States origin scrapie to neonatal sheep.
Scrapie, a transmissible spongiform encephalopathy (TSE), is a naturally occurring fatal neurodegenerative disease of sheep and goats. The current study documents incubation periods, pathologic findings, and distribution of abnormal prion proteins (PrP(Sc)) by immunohistochemistry and Western blot in tissues of genetically susceptible and resistant neonatal lambs inoculated with pooled brain ho...
متن کاملExperimental transmission of US scrapie agent by nasal, peritoneal, and conjunctival routes to genetically susceptible sheep.
Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. This study documents incubation periods, pathologic findings, and distribution of abnormal prion proteins (PrP(Sc)) by immunohistochemistry in tissues of genetically susceptible sheep inoculated with US sheep scrapie agent. Four-month-old Suffolk lambs (QQ at codon 171) were inoculated by 1 of 3 different route...
متن کاملA test of EcoRI and HindIII restriction fragment length polymorphisms in assessing susceptibility for scrapie in US Suffolk sheep
Ž . We investigated the distribution of EcoRI restriction fragment length polymorphism RFLP and tested EcoRI and HindIII RFLPs to ascertain the occurrence of scrapie in Suffolk sheep. Genomic DNA samples were collected from 527 Ž . animals and typed for EcoRI RFLP by Southern blot analysis using the PrP gene clone probe pNPU42 . The average e1 frequency in Suffolk sheep was 64.4% and ranged fro...
متن کاملIntraspecies Prion Transmission Results in Selection of Sheep Scrapie Strains
BACKGROUND Sheep scrapie is caused by multiple prion strains, which have been classified on the basis of their biological characteristics in inbred mice. The heterogeneity of natural scrapie prions in individual sheep and in sheep flocks has not been clearly defined. METHODOLOGY/PRINCIPAL FINDINGS In this study, we intravenously injected 2 sheep (Suffolk and Corriedale) with material from a n...
متن کاملBreeding for resistance to scrapie: Detection of polymorphisms in the prion protein gene in German sheep breeds
Scrapie is a fatal neurodegenerative disease of sheep and goats belonging to the group of transmissible spongiform encephalopathies (TSE). TSEs are characterised by distinctive pathology, the involvement of a hostencoded prion protein (PrP) in disease development and genetic control of susceptibility. Polymorphisms in three different codons of the ovine prion protein (PrP) gene (136, 154, 171) ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of general virology
دوره 78 ( Pt 4) شماره
صفحات -
تاریخ انتشار 1997